Proper use of Transmission-Based Precautions is essential to preventing the spread of infectious diseases within healthcare settings. This collection includes the current Transmission-Based Precautions signage developed by the Centers for Disease Control and Prevention (CDC), providing healthcare personnel with clear, standardized visual reminders for implementing Contact, Droplet, Airborne, Enhanced Barrier, and other applicable precautions. These signs are intended to be posted at the entrance to patient care areas to communicate the personal protective equipment (PPE), isolation requirements, and infection prevention practices necessary to protect patients, visitors, and healthcare personnel. Using standardized CDC signage helps promote consistency, regulatory compliance, and adherence to evidence-based infection prevention practices across all healthcare environments.